Toggle navigation. Mikhail Tswett — inventor of chromatography method. Olympic rings inspires chemical molecule Olympicene. James Batcheller Sumner — discoverer of protein nature of enzymes. John Alexander Reina Newlands — discoverer of periodic table. Mikhail Vasilyevich Lomonosov — Russian polymath. Related Articles 2, proteins present in HIV virus! Related Products. Alcohol View Suppliers. Ethanol View Suppliers. Calcium Carbonate View Suppliers.
Subscribe to our Newsletter Stay updated with the latest chemical industry trends and innovations. Kimberlite Softwares Pvt. All rights reserved. Chromatography is a fundamental technique for the separation, detection, identification and quantitation of chemical species.
It comes in two basic formats: planar and column chromatography. Chromatographic techniques can also be classified according to whether they are being used for identification and measurement analytical , for microscale to trace quantities of analyte or as a purification step preparative , for semimicro to macroscale quantities of analyte.
Today, chromatographic techniques are essential tools in areas such as chemistry, biology, medicine, forensic science, manufacturing, and the environment, and are arguably the most widely used of any family of analytical methods.
The origins of planar chromatography can be traced back to the practice amongst dyers of testing pigments by placing a drop on paper and observing the colours as the drop spreads. The scientific use of paper chromatography can be traced to the mid th century. In planar chromatography, samples and standards are placed along a line at one end of a piece of paper or plate coated with a porous solid layer — the stationary phase.
When trying to identify an unknown substance, the sample must first be simplified as much as possible into its constituent compounds. The unknown can then be characterized by individual identification of its parts. This does not imply that the separated chemical components are recovered after the separation and analyzed. Usually, the analytes are irretrievable. Separated compounds are compared to known standards.
As with most chemical exploration, it is important to have an idea of what compounds are being searched for in the first place.
0コメント